Factors influencing detection of Potato Leafroll Virus and Potato Virus Y in potato tuber extracts
نویسندگان
چکیده
منابع مشابه
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملتعیین برخی خصوصیات بیولوژیکی و مولکولی potato leafroll virus و potato virus a در استان اصفهان
چکیده سیب زمینی یکی از مهم ترین محصولات زراعی در جهان می باشد. ویروس های متعددی این گیاه را تحت تاثیر قرار می دهند و باعث خسارت اقتصادی قابل توجهی می شوند. ویروس پیچیدگی برگ سیب زمینی potato leafroll virus (plrv) عضو تیپ جنس polerovirus و خانواده luteoviridae و ویروس ای سیب زمینی potato virus a (pva) عضو تیپ جنس potyvirus از خانواده potyviridae شایع ترین ویروس های سیب زمینی می باشند که توسط ش...
15 صفحه اولErratum: The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus
[This corrects the article on p. 321 in vol. 32, PMID: 27493607.].
متن کاملHeterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملDevelopment of RT-PCR based method for detection of potato virus Y in tobacco and potato
A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Breeding and Seed Science
سال: 2009
ISSN: 2083-599X,1429-3862
DOI: 10.2478/v10129-009-0005-7